Nonnative species are a key agent of global change. However, nonnative invertebrates remain understudied at the community scales where they are most likely to drive local extirpations. We use the North American NEON pitfall trapping network to docu- ment the number of nonnative species from 51 invertebrate communities, testing four classes of drivers. We sequenced samples using the eDNA from the sample’s...

Amphibians represent one of the most threatened vertebrate groups, and although monitoring amphibian population dynamics is critical for conservation, most traditional survey efforts depend on time-consuming, often invasive monitoring activities and visual surveys. Screening environmental DNA (eDNA), a non-invasive monitoring technique, has the potential to identify species presence at a site, even in the absence of visual confirmation. In this study, we...

We describe the development and validation of a qPCR assay to detect Poecilia reticulata, a highly invasive species of freshwater fish invasive to the Caribbean islands, through environmental DNA (eDNA) sampling. Originating from Trinidad, this species is invasive and detrimental to countless native tropical fish communities. A qPCR assay, consisting of a set of primers and a fluorescent probe, amplifying a 214...